International Journal of Aquaculture, 2026, Vol.16, No.4, 243-281 http://www.aquapublisher.com/index.php/ija 264 3.6.2 Basophils The basophil counts in the fish fed with different immunostimulant mixed feeds under normal and Aeromonas infected conditions are shown in Table 25. Based on these findings, it was determined that there was no statistically significant variation in the average basophil counts across the groups being tested. The average basophil counts in healthy fish treated with different immunostimulants mixed feed ranged from 38±9 to 48±9 cells/mm3. In Aeromonas infected fish, basophil counts with different immunostimulant mixed feeds typically ranged from 34±6.5 to 42.6±6.02 cells/mm3. The mean basophil counts in healthy fish fed with immunostimulant mixed feed such as control, vitamin C, vitamin E, chitin, chitosan, and levamisole was found to be 38±9, 44.3±8.50, 48±9, 38±9.53, 43.3±11.06, and 46.33±8.50 cells/mm3 respectively. As well as, the mean basophil counts in Aeromonas infected fish fed with immunostimulant mixed feed such as control, vitamin C, vitamin E, chitin, chitosan, and levamisole was found to be 34±6.5, 39±8, 42.66±6.02, 36.33±8.50, 39±8.54, and 42.66±11.5 cells/mm3 respectively. Table 25 Basophils count in the fish fed with different immunostimulant mixed feeds under normal and Aeromonas hydrophila infected conditions S. No. Experimental feed Basophils (cells/mm3) F- value p- value Healthy fish Aeromonas Infected fish 1 Control feed 38.0±9 34.0±6.5 0.33 0.596NS 2 Vitamin C mixed feed 44.3±8.50 39.0±8 0.63 0.47 NS 3 Vitamin E mixed feed 48.0±9 42.66±6.02 0.73 0.441NS 4 Chitin mixed feed 38.0±9.53 36.33±8.50 0.05 0.834NS 5 Chitosan mixed feed 43.3±11.06 39.0±8.54 0.29 0.618 NS 6 Levamisole mixed feed 46.33±8.50 42.66±11.5 0.2 0.677NS p-value* = Significant, p-value** = highly significant, p-value*** = extremely significant, p-valueNS = Not Significant The number of basophils insignificantly varies between healthy and Aeromonas infected fish from all the treatment groups. The p - values for the basophil counts between healthy and Aeromonas infected fish in the treatment groups of control, vitamin C, vitamin E, chitin, chitosan, and levamisole were measured as 0.596, 0.471, 0.441, 0.834, 0.618, and 0.677 respectively. These results demonstrated that the tested immunostimulants don’t exhibit a significant impact on the basophil count in both healthy and Aeromonas infected fish when compared to the control control-fed group. 3.6.3 Neutrophils The Table 26 shows the mean neutrophil counts of healthy and Aeromonas infected Catla catla fish that were fed with different immunostimulant-supplemented feeds. From these results, it was shown that the average neutrophils count in all test groups varied significantly. The mean neutrophil counts in healthy fish treated with different immunostimulants mixed feed ranged from 2650±0.525 to 3340±0.49 cells/mm3. Whereas, in Aeromonas infected fish, neutrophil counts with different immunostimulant mixed feeds ranged between 5060±0.89 and 7570±0.62 cells/mm3. Table 26 Neutrophil counts in the fish fed with different immunostimulant mixed feeds under normal and Aeromonas hydrophila infected conditions. S. No. Experimental feed Neutrophils (cells/mm3) F- value p- value Healthy fish Aeromonas infected fish 1 Control feed 2650±0.525 5060±0.89 8.46 0.043* 2 Vitamin C mixed feed 2806±0.62 6890±0.77 51.11 0.0020** 3 Vitamin E mixed feed 3340±0.49 7570±0.62 110.1 0.0005*** 4 Chitin mixed feed 3150±0.625 5640±0.525 28.04 0.0061** 5 Chitosan mixed feed 2860±0.576 6206±0.765 36.6 0.0037** 6 Levamisole mixed feed 2910±0.672 7100±1.030 37.79 0.0041** p-value* = Significant, p-value** = highly significant, p-value*** = extremely significant, p-valueNS = Not Significant
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