International Journal of Aquaculture, 2026, Vol.16, No.4, 243-281 http://www.aquapublisher.com/index.php/ija 265 The mean neutrophil counts in healthy fish fed with immunostimulant mixed feed such as control, vitamin C, vitamin E, chitin, chitosan, and levamisole were found to be 2650±0.525, 2806±0.62, 3340±0.49, 3150±0.625, 2860±0.576, and 2910±0.672 cells/mm3 respectively. As well as, the mean neutrophil number in Aeromonas infected fish fed with immunostimulant mixed feed such as control, vitamin C, vitamin E, chitin, chitosan, and levamisole were found to be 5060±0.89, 6890±0.77, 7570±0.62, 5640±0.525, 6206±0.765, and 7100±1.030 cells/mm3 respectively. The number of neutrophils significantly varies between healthy and Aeromonas infected fish from all the treatment groups. The p - values of the neutrophil counts between healthy and Aeromonas infected fish in the treatment groups of control, vitamin C, vitamin E, chitin, chitosan, and levamisole were measured as 0.043, 0.0020, 0.00046, 0.0061, 0.0037, and 0.0041 respectively. These results demonstrated that the tested immunostimulants significantly increased the neutrophils content in both healthy and Aeromonas infected fish when compared to the control. 3.6.4 Lymphocytes The Table 27, presents the mean lymphocyte counts of healthy and Aeromonas-infected Catla catla fish that were given various immunostimulant-mixed feeds, along with a control feed. Based on these findings, it was noted that the average lymphocyte counts in all the test groups, with the exception of the chitosan mixed diet group, showed significant variation. The mean lymphocyte counts in healthy fish that treated with different immunostimulant mixed feeds ranged from 2531±0.79 to 3072±0.99 cells/mm3. Whereas, in Aeromonas-infected fish, the lymphocyte counts varied between 2290±1.095 and 2740±1.270 cells/mm3 with the use of different immunostimulant mixed feeds. Table 27 Lymphocyte counts in the fish fed with different immunostimulant mixed feeds under normal and Aeromonas hydrophila infected conditions S. No. Experimental feed Lymphocytes (cells/mm3) F - value p – value Healthy fish Aeromonas infected fish 1 Control feed 2531±0.79 2229±1.095 14.92 0.018* 2 Vitamin C mixed feed 2745±0.87 2459±1.046 13.3 0.021* 3 Vitamin E mixed feed 3072±0.99 2740±1.270 12.7 0.023* 4 Chitin mixed feed 2629±0.92 2353±1.084 11.28 0.028* 5 Chitosan mixed feed 2544±1.17 2360±1.06 4.03 0.115 NS 6 Levamisole mixed feed 2850±0.89 2553±1.22 11.6 0.027* p-value* = Significant, p-value** = highly significant, p-value*** = extremely significant, p-valueNS = Not Significant The average lymphocyte counts in healthy fish that were fed with different types of immunostimulant mixed feeds including control, vitamin C, vitamin E, chitin, chitosan, and levamisole, were measured to be 2531±0.79, 2531±0.79, 3072±0.99, 2629±0.92, 2544±1.17, and 2850±0.89 cells/mm3 respectively. As well as, the mean counts of lymphocytes in Aeromonas infected fish that were fed with different types of immunostimulant mixed feeds including control, vitamin C, vitamin E, chitin, chitosan, and levamisole were reported to be 2229±1.095, 2459±1.046, 2740±1.270, 2353±1.084, 2360±1.06, and 2553±1.22 cells/mm3 respectively. The number of lymphocytes shows considerable variation between healthy fish and those infected with Aeromonas across all treatment groups. The p - values for the lymphocyte counts between healthy and Aeromonas infected fish in the treatment groups of control, vitamin C, vitamin E, chitin, chitosan, and levamisole were 0.018, 0.021, 0.023, 0.028, 0.115, and 0.027 respectively. These results indicated that the tested immunostimulants had a substantial effect on increasing significantly the number of lymphocytes in both healthy and Aeromonas infected fish when compared to the control control-fed group.
RkJQdWJsaXNoZXIy MjQ4ODYzNA==