International Journal of Aquaculture, 2026, Vol.16, No.4, 243-281 http://www.aquapublisher.com/index.php/ija 263 The mean total WBC count in healthy fish fed with immunostimulant mixed feed such as control, vitamin C, vitamin E, chitin, chitosan, and levamisole was found to be 4077±0.93, 6855±0.861, 7535±1.07, 6613±0.74, 6322±0.84, and 7248±0.82 cells/mm3 respectively. As well as, the mean total WBC count in Aeromonas infected fish fed with immunostimulant mixed feed such as control, vitamin C, vitamin E, chitin, chitosan, and levamisole were found to be 4342±0.87, 7130±0.43, 7757±1.06, 6849±1.12, 6435±0.92, and 7373±0.95 ×103/mm3 respectively. The total WBC count significantly varies between healthy and Aeromonas infected fish from all the treatment groups. The p - values for the total WBC count content between healthy and Aeromonas infected fish in the treatment groups of control, vitamin C, vitamin E, chitin, chitosan, and levamisole were measured as 0.0228, 0.134, 0.063, 0.034, 0.195, and 0.156 respectively. These results demonstrated that the chitin mixed feed significantly increased the total WBC count content in both healthy and Aeromonas infected fish when compared to the control control-fed group. 3.6 Differential leucocyte count Differential blood counts of the fish fed with different immunostimulant mixed feeds under normal and Aeromonas infected conditions were performed by counting the blood cells including eosinophils, basophils, neutrophils, lymphocytes, and monocytes. 3.6.1 Eosinophils The Table 24 shows the mean eosinophil count of healthy and Aeromonas infected Catla catla fish that were treated with different immunostimulant mixed feeds. According to these results, it was observed that the average eosinophil count in all test groups varied significantly. The average eosinophil count in healthy fish treated with different immunostimulant-supplemented feeds ranged from 357±53.07 to 475±45.76 cells/mm3. In Aeromonas infected fish, eosinophil count with different immunostimulant mixed feeds typically ranges from 369±50.21 to 543.66±52.51 cells/mm3. Table 24 Eosinophil count in the fish fed with different immunostimulant mixed feeds under normal and Aeromonas hydrophila infected conditions S. No. Experimental feed Eosinophils (cells/mm3) F- value p- value Healthy fish Aeromonas infected fish 1 Control feed 475±45.76 543.66±52.51 2.86 0.166NS 2 Vitamin C mixed feed 412±49.31 408±44.65 0.01 0.925NS 3 Vitamin E mixed feed 357±53.07 369±50.21 0.09 0.779NS 4 Chitin mixed feed 417±53.02 454±52.003 0.77 0.421NS 5 Chitosan mixed feed 430±47.12 466±46.36 0.91 0.394NS 6 Levamisole mixed feed 358±41.06 393±67.002 0.61 0.47NS p-value* = Significant, p-value** = highly significant, p-value*** = extremely significant, p-valueNS = Not Significant The mean eosinophil count in healthy fish fed with immunostimulant mixed feeds such as control, vitamin C, vitamin E, chitin, chitosan, and levamisole was found to be 475±45.76, 412±49.31, 357±53.07, 417±53.02, 430±47.12, and 358±41.06 cells/mm3 respectively. As well as, the mean eosinophil count in Aeromonas infected fish fed with immunostimulant mixed feed such as control, vitamin C, vitamin E, chitin, chitosan, and levamisole was found to be 543.66±52.51, 408±44.65, 369±50.21, 454±52.003, 466±46.36, and 393±67.002 cells/mm3 respectively. The number of eosinophils significantly varies between healthy and Aeromonas infected fish from all the treatment groups. The p - values for the eosinophil count healthy and Aeromonas infected fish in the treatment groups of control, vitamin C, vitamin E, chitin, chitosan, and levamisole were measured as 0.166, 0.925, 0.779, 0.421, 0.394, and 0.47 respectively. These results demonstrated that the tested immunostimulants decreased eosinophil count but not significantly in both healthy and Aeromonas infected fish when compared to the control control-fed group.
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