International Journal of Aquaculture, 2026, Vol.16, No.4, 243-281 http://www.aquapublisher.com/index.php/ija 262 3.4 Total RBC count The mean total RBC count in the fish fed with different immunostimulant mixed feeds under normal and Aeromonas infected conditions is shown in Table 22. These results demonstrated significant variation in the mean values of the total RBC count across all test groups. The average total RBC count in healthy fish treated with different immunostimulant-supplemented feeds ranged from 1.24±0.08 to 2.85±2.09 ×106/mm3. In Aeromonas infected fish, total RBC count with different immunostimulant mixed feeds typically varies from 1.16±0.05 to 2.25±0.08×106/mm3. Table 22 Total RBC count in the fish fed with different immunostimulant mixed feeds under normal and Aeromonas hydrophila infected conditions S. No. Experimental feed Mean Total RBC count (×106/mm3) F – value p – value Healthy fish Aeromonas infected fish 1 Control feed 1.24±0.08 1.16±0.05 2.41 0.196NS 2 Vitamin C mixed feed 2.59±0.05 2.05±0.07 121.13 0.0004*** 3 Vitamin E mixed feed 2.85±0.09 2.25±0.08 73.64 0.001*** 4 Chitin mixed feed 1.67±0.06 1.26±0.05 99.53 0.0006*** 5 Chitosan mixed feed 1.77±0.06 1.33±0.04 109.49 0.0005*** 6 Levamisole mixed feed 2.21±0.03 1.78±0.05 214.51 0.0001*** p-value* = Significant, p-value** = highly significant, p-value*** = extremely significant, p-valueNS = Not Significant The mean total RBC count in healthy fish fed with immunostimulant mixed feed such as control, vitamin C, vitamin E, chitin, chitosan, and levamisole was found to be 1.24±0.08, 2.59±0.05, 2.85±0.09, 1.67±0.06, 1.77±0.06, and 2.21±0.03×106/mm3 respectively. As well as, the mean total RBC count in Aeromonas infected fish fed with immunostimulant mixed feed such as control, vitamin C, vitamin E, chitin, chitosan, and levamisole was found to be 1.16±0.05, 2.05±0.07, 2.25±0.08, 1.26±0.05, 1.33±0.04, and 1.78±0.05×106/mm3 respectively. The total RBC count significantly varies between healthy and Aeromonas infected fish from all the treatment groups. The p - values for the total RBC count between healthy and Aeromonas infected fish in the treatment groups of control, vitamin C, vitamin E, chitin, chitosan, and levamisole were measured as 0.196, 0.0004, 0.001, 0.0006, 0.0005, and 0.0001 respectively. These results demonstrated that the tested immunostimulants significantly increased the total RBC count in both healthy and Aeromonas infected fish when compared to the control control-fed group. 3.5 Total WBC count The mean total WBC count in the fish fed with different immunostimulant mixed feeds under normal and Aeromonas infected conditions is shown in Table 23. From these results, it was observed that the mean WBC count in all test groups varied significantly. The average total WBC count in healthy fish treated with different immunostimulant-supplemented feeds ranged from 4077±0.93 to 7535±1.07 cells/mm3. In Aeromonas infected fish, the total WBC count with different immunostimulant mixed feeds typically ranges from 4342±0.87 to 7757±1.06 cells/mm3. Table 23 Total WBC count in the fish fed with different immunostimulant mixed feeds under normal and Aeromonas hydrophila infected conditions S. No. Experimental feed Mean Total WBC count (cells/mm3) F- value p – value Healthy fish Aeromonas infected fish 1 Control feed 4077±0.93 4342±0.87 12.94 0.0228* 2 Vitamin C mixed feed 6855±0.86 7130±0.43 17.43 0.134NS 3 Vitamin E mixed feed 7535±1.07 7757±1.06 6.5 0.063NS 4 Chitin mixed feed 6613±0.74 6849±1.12 9.1 0.034* 5 Chitosan mixed feed 6322±0.84 6435±0.92 2.41 0.195NS 6 Levamisole mixed feed 7248±0.82 7373±0.95 2.97 0.156NS p-value* = Significant, p-value** = highly significant, p-value*** = extremely significant, p-valueNS = Not Significant
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