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Intl. J. of Molecular Zoology, 2012, Vol.2, No.1, 1
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Figure 6 Mean values ± SD of the transglutaminase (g/L) in the
studied groups
with bovine colostrum inhibited the increased level of
transglutaminase in autistic pups with Coeliac disease.
1.3.6 Mean calcium (mg/dl)
The calcium level was significantly decreased in
group 1 as compared to normal control (7.31±0.411
mg/dL vs 10.91±0.241 mg/dL) (P<0.05), respectively.
Furthermore, significantly increased in group 2 as
compared to group 1 (9.71±0.325 vs 7.31±0.41). No
significant difference of mean calcium level was
observed between group 2 and normal control groups
(
P
>0.05). This means that bovine colostrums
treatment was more effective than mother's milk
treatment in increasing calcium level as evidenced by
the detected significant changes between the two
groups (Figure 7).
Figure 7 Mean values ± SD of total calcium (mg/dl) in the
studied groups
1.4 Gene expression in different studied groups
1.4.1
Met
gene expression in study 1 on PND0
The expression of
Met
gene shows approximately
6.5 fold up regulated. After normalization
Met
gene was 5.5 up regulated compared to control as
shown in Figure 8.
1.4.2
Met
and
DQ2
genes expression in study 2
(group 1 and group 2) on PND21
As shown in Figure 9, the expression of
Met
and
DQ2
Figure 8 The expression of
Met
gene in control and study 1
genes shows in Group I, 5.0 and 4.2 fold expression
respectively. After normalization 4.0 and 3.2 fold expression
of
Met
and
DQ2
gene compared to control respectively. In
Group II, gene expression of
DQ2
and
Met
gene were
1.5 and 1.3 respectively. Whereas, after normalization
of
DQ2
and
Met
gene were 0.5 and 0.3 respectively.
Figure 9 The expression of
Met
and
DQ2
genes in control,
group1 and group 2
1.5 Scanning electron microscopy of the duodenal villi
on PND 21 from the following groups:
1.5.1 Control group
The scanning electron microscope demonstrated the
duodenal villi with intact tips and borders. The
openings of goblet cells were observed on the surface
of the villi (Figure 10).
Figure 10 A scanning electron micrograph of dudodenal villi of
21
-
old pups of control showing the villi with intact tips and
border (
↓
), notice the opening of goblet cells on the surface of
the villi ( ) ( x 330)