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Intl. J. of Molecular Zoology, 2012, Vol.2, No.1, 1
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Table 2 Changes of biochemical parameters in control and study 1 on PND 0 (mean ± S.D.)
Note: * P<0.05, ** P<0.01, compared with control group
Table 3 Changes of biochemical parameters in control and study 2 on PND 21 (mean ± S.D.)
Note: * P<0.05, ** P<0.01, compared with control group; # P<0.05, ## P<0.01, compared with group 1.
1.3.3 Mean epinephrine (ng/L)
As shown also in Table 3, Figure 4 mean epinephrine
was significantly increased (P<0.001) in all treated
groups whether in group 1 (Suckling pups + gliadin),
group 2 (bovine colostrums + gliadin) (on PND 21)
as compared to controls (76.22±1.15) ng/L,
(35.29±338) ng/L vs (21.73±290) ng/L, respectively.
But epinephrine level was significantly (P<0.001)
decreased in group 2 treated pups as compared to
group 1 treated pups (35.29±0.338) vs (76.22±1.15).
Figure 4 Mean values ± SD of epinephrine (ng/L) in the studied
groups
1.3.4 Mean norepinephrine (ng/L)
As shown in Table 3, Figure 5 mean norepinephrine was
significantly increased (P<0.001) in all treated groups
whether in group 1 (Suckling pups + gliadin) and
2
(bovine colostrums + gliadin) (on PND 21) as compared
to controls (204.87±1.83) ng/L, (183.72±1.42) ng/L,
Figure 5 Mean values ± SD of norepinephrine (ng/L) in the
studied groups
vs 110.46±0.990) ng/L, respectively. Moreover, no
significant change was detected in group 2 treated
pups as compared to group 1 treated pups (p>0.05).
1.3.5 Mean transglutaminase (g/L)
As shown also in Table 3, Figure 6 mean
transglutaminase was significantly increased (P<0.001)
in group 1 (Suckling pups + gliadin) (on PND 21) as
compared to controls (3.1±0.149 g/L vs 0.9±0.066 g/L,
respectively). Furthermore, mean transglutaminase level
was significantly (P<0.001) decreased in group 2 treated
pups as compared to group 1 treated control. Moreover,
no significant change was detected in group 2 treated
pups as compared to normal control pups. Treatment
Biochemical parameters vit D
Serotonin
Epinephrine
Nor epinephrine
Calcium
Unit
ng/mL
ug/L
ng/L
ng/L
mg/dL
Method
Groups
CMIA
HPLC
HPLC
HPLC
Calorimetric
Control
34.26±0.514
7.26±0.604
16.54±0.185
101.32±1.683
10.01±0.211
Study 1
6.21±0.13
**
33.3±0.244
**
84.25±595
**
184.29±8.44
**
6.52±0.169
**
Parameters Vit D
Serotonin
Epinephrine
Norepinephrine
Transglutaminase
Calcium
Unit
ng/mL
ug/L
ng/L
ng/L
g/L
mg/dL
Method
Groups
CMIA
HPLC
HPLC
HPLC
ELISA
Calorimetric
Control
44.36±0.302
9.12±0.126
21.73±0.290
110.46±0.990
0.9±0.066
10.91±0.241
Group 1
9.4±0.225
**
30.5±0.565
**
76.22±1.15
**
204.87±1.83
**
3.1±0.149
**
7.31±0.411
*
Group 2
41.16±0.622
*##
12.55±0.297
*##
35.29±0.338
**##
183.72±1.42
**
1.1±0.101
##
9.71±0.325
#