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Intl. J. of Molecular Zoology, 2012, Vol.2, No.1, 1
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Figure 1 Mean values ± SD of the mean body weights (g) in the
studied groups
(Suckling pups + gliadin) showed a statistically
significantly (
P
<0.001) reduced increase in body
weight till weaning in comparison with normal control
(21.40±2.36) g vs (33.4±2.7) g. While significant
increase (
P
<0.001) in body weight was detected in
bovine colostrums treated pups on PND 7 and 21 as
compared to suckling pups in group 1.But no
significant difference was noticed between the two
groups on PND 0 and 14. The animals showed long
bones malformation on PND 0 and the stools were
sticky &loose in group 1 on PND 14 (results not showed).
1.2 Changes of biochemical parameters in control
group and study 1 on PND 0
Table 2 shows the changes of biochemical parameters
of control groups and autistic pups. The mean change
in study 1 group was significantly increased in
epinephrine, norepinephrine and serotonin compared
with that in control group (P<0.001). Mother
administered a single intraperitoneal injection of
600 mg/kg sodium valproate on day 12.5 after
conception results in significantly reduced calcium
and Vit D levels in pups (study 1) as compared with
normal control groups (P<0.001).
1.3 Changes of biochemical parameters in control
and study 2 on PND 21 (mean±S.D.)
Considering the key roles of gliadin in the
development of coeliac disease in autistic pups, it was
necessary and important to investigate the changes of
the chemical parameters for Coeliac disease
concomitant with autism, which were displayed in
Table 3. Almost all the above adverse effects induced
by autism in Table 2 were suppressed by bovine
colostrums treatment in group 2 as follows:
Figure 2 Mean values ± SD of the mean Vit D (ng/mL) in the
studied groups
1.3.1 Mean vitamin D (Vit D) (ng/mL)
As shown in Table 3, Figure 2, significant difference
of vit D level was observed between group 2 and
normal control groups (p<0.05) (41.16±0.622)
ng/mL vs (44.36±0.302) ng/mL, respectively).
Furthermore, 21-day-old rat pups, group 1 (suckling
pups + gliadin) significantly lowered the mean vit D
level as compared to normal control group (p<0.001)
(9.4± 0.225) vs (44.36±0.302). While treatment
with bovine colostrums significantly increased vit D
levels (p<0.001) as compared to suckling pups in
group 1. Treatment with bovine colostrum inhibited
the decreased level of vit D in autistic pups with
Coeliac disease.
1.3.2 Mean serotonin (μg/L)
As shown in Table 3, Figure 3, a significant increase
in mean serotonin levels was detected in group 1
treated control, as compared to normal control pups
(30.5±0.565) vs (9.12±0.126) (p<0.001). While a
significant decrease in serotonin level was detected in
group 2 pups treated with bovine colostrums as
compared to group 1, (12.55±0.2976) ug/L vs
(30.5±0.565) ug/L respectively (p<0.001).
Figure 3 Mean values ± SD of serotonin (μg/L) in the studied
groups