Plant Gene and Trait 2026, Vol.17, No.1, 1-11 http://genbreedpublisher.com/index.php/pgt 5 The clustering analysis of Platycladus orientalis (Figure 3) showed that red represents Cluster I, blue represents Cluster II, and yellow represents Cluster III. Cluster I contained 13 individuals, including 7 from Shanting District, 4 from Tengzhou City, and 1 from Yicheng District. Cluster II contained 23 individuals, including 21 from Shanting District and 2 from Shizhong District. Cluster III contained 64 individuals, including 40 from Shanting District, 15 from Tengzhou City, 8 from Yicheng District, and 1 from Shizhong District. Figure 3 Phylogenetic tree of Platycladus orientalis based on SSR data Using NTSYS software, UPGMA clustering analysis based on Nei’s genetic distance was performed on 100 samples from four populations (Figure 4). Coefficient 0.69 0.77 0.85 0.92 1.00 118 118 66 102 117 161 24 75 196 4 135 81 116 167 3 72 119 43 169 185 28 176 8 63 5 78 11 76 96 199 200 150 168 158 47 120 170 178 2 25 35 36 52 103 157 113 179 37 187 145 27 64 89 148 83 98 182 59 80 85 122 183 39 193 156 155 166 13 74 100 137 197 159 10 56 109 53 68 99 106 107 54 127 124 152 189 141 49 50 7 70 174 26 32 55 38 144 151 9 115 195 Figure 4 Dendrogram of Platycladus orientalis based on SSR data At a similarity coefficient of approximately 0.69, the samples were divided into two clusters. Samples 135, 137, 141, and 145 collected from Shizhong District, which showed relatively close kinship, were grouped together with samples from other regions, while samples from the remaining regions were not strictly clustered according to their geographic origins. At a similarity coefficient of approximately 0.70, four clusters were identified, and the collected samples still did not cluster according to the four sampling regions. When the genetic similarity coefficient reached 0.77, the 100 Platycladus orientalis accessions were divided into eight clusters.
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