Plant Gene and Trait 2025, Vol.16, No.1, 39-46 http://genbreedpublisher.com/index.php/pgt 40 2 Growth and Metabolic Characteristics of Anoectochilus roxburghii Tissue Culture Seedlings 2.1 Current status and technical applications of tissue culture inAnoectochilus roxburghii seedlings Tissue culture has become a key method for the propagation and protection of Anoectochilus roxburghii. The research by Xu et al. (2016) found that lighting with LED lights can promote the growth of Anoectochilus roxburghii seedlings and also increase the accumulation of flavonoids. The light source combining blue and red light (BR LED) is more effective than other lighting conditions in increasing plant height, stem thickness, leaf number and flavonoid content, and is regarded as a promising in vitro propagation technique. The research conducted by Zhang et al. in 2020 demonstrated that optimizing the formula of the culture medium could also enhance the induction, proliferation and regeneration efficiency of the protocorm-like body (PLBs), thereby increasing the success rate of micropropagation of Anoectochilus roxburghii. 2.2 Dynamic changes in major growth indicators of Anoectochilus roxburghii The research by Wang et al. (2018) found that the seedlings of the Anoectochilus roxburghii grew better under the combined lighting of blue and red light (BR LED), with taller plants, thicker stems and larger leaf areas (Figure 1). Li et al. (2017) demonstrated that some endophytic fungi, such as Chaetomium globosum and Colletotrichum gloeosporioides, could promote the growth of plants. They can not only increase biomass, but also regulate the expression of genes related to plant growth. These research results indicate that environmental conditions and beneficial microorganisms play a very important role in the cultivation of Anoectochilus roxburghii. Figure 1 Cultivation of Anoectochilus roxburghii under supplemental lighting with various light qualities (Adopted from Wang et al., 2018) Image caption: A: Red light; B: Blue light; C: Yellow light; D: Green light; E: White light; F: Control (40 days) (Adopted from Wang et al., 2018)
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