Bioscience Evidence 2026, Vol.16, No.4, 291-303 http://bioscipublisher.com/index.php/be 298 Recent studies further confirmed the advantages of Guizhou’s blueberry production environment. Blueberries grown in the karst regions of Guizhou showed significantly higher anthocyanin content than fruits from other production areas, and Deng et al. (2026) identified the involvement of the VdMYB56-VdMYB69 regulatory module in anthocyanin accumulation. The combination of favorable ecological conditions and suitable genetic backgrounds promotes the expression of anthocyanin biosynthesis-related genes, contributing to the high functional nutritional value of Guizhou blueberries (Deng et al., 2026). In addition to field production advantages, Guizhou has also developed effective postharvest preservation technologies. Wu et al. (2022) investigated the effects of ozone fumigation on postharvest quality of blueberries from Majiang County, Guizhou Province. During storage at 4 °C, ozone treatment significantly reduced fruit decay, delayed the decline of total phenolics and anthocyanins, and maintained higher antioxidant enzyme activity. After 80 days of storage, ozone-treated fruit retained higher soluble solids, total phenolic content, and anthocyanin levels than untreated fruit, demonstrating that appropriate postharvest technologies can effectively preserve blueberry nutritional quality. 5.3 Liaoning province Liaoning Province is an important region for blueberry breeding and protected cultivation in northern China, with extensive experience in cultivar evaluation and high-quality fruit production. Akbari et al. (2026) systematically evaluated 21 highbush blueberry cultivars (Vaccinium corymbosum L.) under uniform greenhouse conditions at the Liaoning Institute of Pomology. Under the same environmental conditions, significant differences were observed among cultivars in total phenolics, anthocyanins, sugar content, and antioxidant capacity. Among the evaluated cultivars, ‘Eureka’, ‘Twilight’, and ‘Rossini’ showed particularly high anthocyanin accumulation, while some other cultivars exhibited higher total phenolic content or more favorable sugar-acid balance. Because the experiment was conducted under controlled greenhouse conditions, environmental variation was largely minimized, making the results valuable for cultivar selection and production planning in northern protected cultivation systems. For commercial blueberry production in Liaoning, cultivars can be selected according to different market objectives, such as high anthocyanin content for functional food applications, high phenolic content for antioxidant products, or improved flavor characteristics for fresh markets. This provides a basis for differentiated production strategies in northern China. 5.4 South China production area The South China blueberry production area mainly includes Guangdong, Guangxi, and Fujian provinces. These regions are characterized by high temperatures, abundant rainfall, and high humidity, and blueberry production commonly relies on substrate culture, container cultivation, and protected cultivation systems. Southern highbush blueberry and rabbiteye blueberry are the major cultivated types in these regions. Therefore, blueberry production in South China is mainly based on cultivation systems adapted to subtropical climatic conditions rather than a single regional model. Chai et al. (2021) systematically analyzed 74 blueberry cultivars collected from southern China and identified 14 anthocyanin compounds. Malvidin, delphinidin, and petunidin were the major contributors to total anthocyanin content, while malvidin-3-O-galactoside was the most abundant individual anthocyanin compound. The study also showed that rabbiteye blueberry (Vaccinium virgatumAiton) generally had higher total anthocyanin content than other blueberry groups, indicating strong genetic differences in anthocyanin accumulation capacity among blueberry types. Nutrient management is also a key factor for southern blueberry production. Anwar et al. (2024) conducted a substrate culture experiment at South China Agricultural University to evaluate the effects of different ammonium nitrogen (NH₄⁺-N) and nitrate nitrogen (NO₃⁻-N) ratios on blueberry growth and fruiting (Figure 3). The results showed that nitrogen form significantly affected plant growth, nutrient uptake, physiological characteristics, flowering, and fruit set. A 50:50 NH₄⁺-N:NO₃⁻-N ratio produced the best overall vegetative growth and fruit-setting performance, whereas a 25:75 ratio was more favorable for soluble sugar accumulation and fruit
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