Molecular Pathogens 2024, Vol.15, No.5, 219-226 http://microbescipublisher.com/index.php/mp 222 RNAi include its specificity, environmentally friendly nature, and potential to reduce the reliance on chemical pesticides (Halder et al., 2022; Koeppe et al., 2023). 4.2.2 Case studies of RNAi in strawberry In strawberries, RNAi has been employed to enhance disease resistance by creating RNAi intragenic silencing cassettes. These cassettes combine specific strawberry promoters with pathogen defense-related DNA sequences to silence corresponding endogenous genes during fruit ripening, thereby improving fruit quality and resistance to pathogens (Figure 2) (Súnico et al., 2021). Additionally, RNAi-based technologies have been explored for their potential to control plant viruses through the exogenous application of double-stranded RNAs (dsRNAs), which can induce systemic RNA silencing and provide a sustainable method for crop protection (Taliansky et al., 2019). These case studies highlight the versatility and effectiveness of RNAi in developing disease-resistant strawberry cultivars. Figure 2 Transient promoter probe analysis of the synthetic FvDOF2 and FvAAT2 DNA fragments, respectively, in F. × ananassa fruit (Adopted from Súnico et al., 2021) Image caption: Numbers 1 and 2 represent two different fruit samples. Histochemical GUS staining was performed after 5 days of fruit infiltration with agrobacterium carrying different query promoter constructs (pFvDOF2: GUS, pFvAAT2: GUS, and pCaMV35s: GUS) or the pKGWFS7.0 empty vector as a negative control (Ø). Query promoter constructs were injected in one half of the fruit, whereas the empty vector (Ø) was injected in the opposite half. For each construct, images represent tissues slices from two different fruit samples (Adopted from Súnico et al., 2021)
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