Molecular Microbiology Research, 2025, Vol.15, No.2, 69-81 http://microbescipublisher.com/index.php/mmr 78 The combined application of biocontrol bacteria and disease-resistant varieties is forming a new trend of complementary synergy. Some experimental results show that applying biocontrol agents on the basis of existing resistant varieties can further improve the level of disease suppression. In some cases, the disease index can be reduced by more than 30%, and the frequency of chemical pesticide use can be reduced (Murmu et al., 2023). This is not only conducive to the multi-pathway play of disease resistance mechanisms, but also in line with the current development direction of sustainable agriculture. Of course, the current industrialization of biological control products still faces challenges, such as the poor stability of some strains under different ecological conditions and the harsh storage and transportation conditions have not been completely solved (Lahlali et al., 2022). In the future, it is necessary to strengthen the research on efficient strain screening, local domestication and supporting preparation technology. At the same time, with the development of synthetic biology and microbiome engineering, compound biocontrol agents designed based on different functional strain combinations may become an important breakthrough for the supporting application of disease-resistant varieties. 6.2 Synergy between agronomic measures and variety resistance Agronomic management measures are also indispensable in the cotton disease prevention and control system, especially in the early stage of disease-resistant variety promotion or in the years with high incidence of diseases, their importance is more prominent. Practice has shown that reasonable crop rotation, intercropping, deep tillage and land preparation, scientific fertilization and other measures can help inhibit the reproduction and spread of pathogens (Zhao et al., 2023b). Crop rotation is one of the most direct and effective methods for preventing and controlling Verticilliumwilt and Fusariumwilt. Rotating with non-host crops such as corn, wheat, and vegetables for 2~3 years can significantly reduce the density of pathogen spores in the soil (Maryum et al., 2022). Studies have shown that after the implementation of the cotton-corn rotation system in the diseased areas of Xinjiang, the incidence of the disease decreased by more than 80%, and the development of the disease was significantly slowed down. Intercropping and green manure planting also have the function of regulating pathogen microecology. For example, intercropping cotton fields with garlic, onions and other onion crops can inhibit the reproduction of pathogens and optimize the structure of rhizosphere microorganisms, thereby indirectly improving cotton's disease resistance (Xiao et al., 2022). In addition, planting green manure crops such as alfalfa and rapeseed and turning them over and returning them to the fields not only improves the physical and chemical properties of the soil, but also helps to enhance the activity of antagonistic microorganisms in the soil. In the cultivation process, moderate density planting, reasonable water and fertilizer regulation, and cleaning of diseased and residual bodies are also the key to ensuring that disease-resistant varieties can give full play to their genetic advantages. High-density planting, excessive ground humidity or excessive nitrogen application may induce the aggravation of Verticillium wilt (Puia et al., 2021). Precision fertilization, combined with potassium supplementation and medium and trace element nutrition management, can help enhance the cotton plant's own defense ability (Lahlali et al., 2022). 7 Application Prospects and Research Trends 7.1 Integration of multi-disease resistance breeding strategies The main direction of cotton disease resistance breeding has shifted from single disease resistance to multi-disease broad-spectrum resistance. Since common diseases in the field do not occur alone, but show a complex epidemic trend, such as Verticilliumwilt, Fusariumwilt, leaf spot, etc., breeding for multi-resistance traits is more practical (Aini et al., 2022). The key to achieving multi-disease resistance breeding lies in the integration of multi-source disease resistance genes. In recent years, through backcrossing, aggregation, molecular marker-assisted selection (MAS) and other means, researchers have gradually integrated traits such as resistance to Verticilliumwilt, resistance to Fusarium
RkJQdWJsaXNoZXIy MjQ4ODYzNA==