· Molecular Microbiology Research, 2025, Vol.15, No.1, 37-44 http://microbescipublisher.com/index.php/mmr 43 soil and allow soybeans to grow faster, indicating that combined inoculation can reduce the amount of fertilizer used. In addition, the experimental results also tell us that different soy varieties need different strains to match. In other words, you cannot choose any bacteria and inoculate it, but choose the most suitable soybeans (Nsengiyumva et al., 2017). 7.3 Policy and market promotion To make these methods really work, not only rely on technology, but also policy support and marketing promotion. If everyone is encouraged to replace nitrogen fertilizer with rhizobia, it will not only reduce environmental pollution, but also make agriculture more sustainable. The country can support relevant scientific research work and promote methods and technologies that have been proven effective, so that more farmers can understand the benefits of rhizobia. By providing subsidies or rewards to farmers, they can also make them more willing to try these new methods. If these measures can be written into agricultural policies, they can help soybean cultivation be more efficient and environmentally friendly, while also increasing yields and ensuring food security (Hasanah et al., 2023). Acknowledgments I would like to express my gratitude to Professor Li M. for his assistance and support throughout the entire research process, as well as to the peer review experts for their opinions Conflict of Interest Disclosure The author affirms that this research was conducted without any commercial or financial relationships that could be construed as a potential conflict of interest. References Allito B.B., Ewusi-Mensah N., and Logah V., 2020, Legume-Rhizobium strain specificity enhances nutrition and nitrogen fixation in faba bean (Vicia faba L.), Agronomy, 10(6): 826. https://doi.org/10.3390/agronomy10060826 Bender F.R., Alves L.C., Da Silva J.F.M., Ribeiro R.A., Pauli G., Nogueira M.A., and Hungria M., 2022, Microbiome of nodules and roots of soybean and common bean: searching for differences associated with contrasting performances in symbiotic nitrogen fixation, International Journal of Molecular Sciences, 23(19): 12035. https://doi.org/10.3390/ijms231912035 Chen L., Qin L., Zhou L., Li X., Chen Z., Sun L., Wang W., Lin Z., Zhao J., Yamaji N., Ma J., Gu M., Xu G., and Liao H., 2018, A nodule-localized phosphate transporter GmPT7 plays an important role in enhancing symbiotic N2 fixation and yield in soybean, The New Phytologist, 221(4): 2013-2025. https://doi.org/10.1111/nph.15541 Cheng Z.Y., Meng L.B., Yin T.J., Li Y., Zhang Y.H., and Li S.M., 2023, Changes in soil rhizobia diversity and their effects on the symbiotic efficiency of soybean intercropped with maize, Agronomy, 13(4): 997. https://doi.org/10.3390/agronomy13040997 Contador C.A., Lo S.K., Chan S.H.J., and Lam H.M., 2020, Metabolic analyses of nitrogen fixation in the soybean microsymbiont Sinorhizobium fredii using constraint-based modeling, mSystems, 5(1): 10.1128. https://doi.org/10.1128/mSystems.00516-19 Dwivedi S., Sahrawat K., Upadhyaya H., Mengoni A., Galardini M., Bazzicalupo M., Biondi E., Hungria M., Kaschuk G., Blair M., and Ortiz R., 2015, Advances in host plant and Rhizobium genomics to enhance symbiotic nitrogen fixation in grain legumes, Advances in Agronomy, 129: 1-116. https://doi.org/10.1016/BS.AGRON.2014.09.001 giehon N.O., Babalola O.O., and Aremu B.R., 2019, Genomic insights into plant growth promoting rhizobia capable of enhancing soybean germination under drought stress, BMC Microbiology, 19: 1-22. https://doi.org/10.1186/s12866-019-1536-1 Hasanah Y., Hanum H., Harahap N.A., and Harahap A.S., 2023, The role of molybdenum in relation to Rhizobium sp., in increasing biological nitrogen fixation and soybean growth, IOP Conference Series: Earth and Environmental Science, 1241(1): 012033. https://doi.org/10.1088/1755-1315/1241/1/012033 He C., Gao H., Wang H., Guo Y., He M., Peng Y., and Wang X., 2020, GSK3-mediated stress signaling inhibits legume-Rhizobium symbiosis by phosphorylating GmNSP1 in soybean, Molecular Plant, 14(3): 488-502. https://doi.org/10.1016/j.molp.2020.12.015 Holland B.L., Matthews M.L., Bota P., Sweetlove L.J., Long S.P., and diCenzo G.C., 2023, A genome-scale metabolic reconstruction of soybean and Bradyrhizobium diazoefficiens reveals the cost-benefit of nitrogen fixation, The New Phytologist, 240(2): 744-756. https://doi.org/10.1111/nph.19203
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