Molecular Microbiology Research, 2025, Vol.15, No.1, 1-9 http://microbescipublisher.com/index.php/mmr 8 problem, it will be difficult to improve the reliability and predictability of genetically modified plants. How to go in the future? In addition to improving conversion efficiency and editing accuracy, a better screening system is also a point worth working on. Looking further away, the potential of cucumber in vaccine carriers and biomaterial production may also be a new way to explore. The concept of sustainable agriculture is quite big, but often it starts with a little technological breakthrough. Agrobacterium rhizogenes mediated gene transformation is one of the directions that make people see the changes. It doesn’t solve all problems out of thin air, but it does play an increasingly role in improving crop adaptability, nutrition and disease resistance. Genetically modified cucumbers, which are resistant to insects and diseases, are a very typical example. After making it, the number of pesticides used can be significantly reduced, and the burden on the environment is also much lighter. This approach is indeed more sustainable than relying on chemical control alone. In addition, some people may have overlooked that it is not just a matter of "farming". Research is using plants to produce recombinant proteins and other biologically active substances, using this transformation system. This alternative production method is not only energy-saving, but also more environmentally friendly than traditional industrial processes. Although it is not a perfect solution, it is certain that as long as this technology continues to move forward, there is still a lot of room for it to play in dealing with major issues such as food security and environmental pressure. Acknowledgments I express my sincere thanks to the Biotechnology Research Center of Cuixi Academy of Biotechnology for their provision of essential resources for this research. I also appreciate the reviewers for their valuable recommendations, which helped in improving the manuscript. Conflict of Interest Disclosure The author affirms that this research was conducted without any commercial or financial relationships that could be construed as a potential conflict of interest. Reference Bhatt R., Asopa P.P., Jain R., Kothari-Chajer A., Kothari S.L., and Kachhwaha S., 2021, Optimization of Agrobacterium mediated genetic transformation in Paspalum scrobiculatum L. (Kodo Millet), Agronomy, 11(6): 1104. https://doi.org/10.3390/AGRONOMY11061104 Chai L.A., Du C.X., Fan H.F., Liu C., and Si Y.Y., 2020a, Improved Agrobacterium tumefaciens-mediated transformation of cucumber via modified use of antibiotics and acetosyringone, Research Square, 2020: 1-28. https://doi.org/10.21203/rs.3.rs-38940/v1 Chai L., Fan H., Liu C., and Du C., 2020b, The progress of transgenic cucumber mediated by Agrobacterium tumefaciens, Trends in Horticulture, 3(1): 93-101. https://doi.org/10.24294/th.v3i1.1791 Choi J., Shin J., Chung Y., and Hyung N., 2012, An efficient selection and regeneration protocol for Agrobacterium-mediated transformation of oriental melon (Cucumis melo L. var. makuwa), Plant Cell Tissue and Organ Culture (PCTOC), 110: 133-140. https://doi.org/10.1007/s11240-012-0137-6 Fan Y., Xu F., Zhou H., Liu X., Yang X., Weng K., Sun X., and Lyu S., 2020, A fast simple high efficient and one-step generation of composite cucumber plants with transgenic roots by Agrobacterium rhizogenes-mediated transformation, Plant Cell Tissue and Organ Culture (PCTOC), 141: 207-216. https://doi.org/10.1007/s11240-020-01781-x Gao D.J., Zhang Q., Xu T.B., Zhou P., Cheng W.J., and Zhang W.W., 2024, Bioinformatics identification and expression profiles of SBP family genes in cucumber (Cucumis sativus L.), Plant Gene and Trait, 15(1): 8-14. https://doi.org/10.5376/pgt.2024.15.0002 Gelvin S., 2010, Plant proteins involved in Agrobacterium-mediated genetic transformation, Annual Review of Phytopathology, 48: 45-68. https://doi.org/10.1146/annurev-phyto-080508-081852 Hasan N., Kamruzzaman M., Islam S., Hoque H., Bhuiyan F.H., and Prodhan S.H., 2019, Development of partial abiotic stress tolerant Citrus reticulata Blanco and (Citrus sinensis L.) osbeck through Agrobacterium-mediated transformation method, Journal of Genetic Engineering and Biotechnology, 17(1): 14. https://doi.org/10.1186/s43141-019-0014-3 Křenek P., Šamajová O., Luptovčiak I., Doskočilová A., Komis G., and Šamaj J., 2015, Transient plant transformation mediated by Agrobacterium tumefaciens: principles methods and applications, Biotechnology Advances, 33(6): 1024-1042. https://doi.org/10.1016/j.biotechadv.2015.03.012
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