Medicinal Plant Research 2026, Vol.16, No.1, 1-10 http://hortherbpublisher.com/index.php/mpr 6 Figure 6 Effect of different concentrations of Tridax procumbens on disease severity in orange fruits pre-inoculated with Lasiodiplodia sp and stored for 30 days 2.1 Phytochemical compositions of the aqueous extract of Tridax procumbens Tannins, alkaloids, cardiac glycosides, saponins and flavonoids were all detected in the Tridax extracts (Table 1). Table 1 Phytochemical constituents in Tridax procumbens Phytochemicals Aqueous extract Tannins + Alkaloids + Cardiac glycosides + Saponins + Flavonoids + 2.2 Bioactive compounds detected in aqueous Tridax procumbens extracts Table 2 shows the bioactive compounds with their peak area (%) detected in Tridax extract included 2,3-Butanediol (10.05), Hexanoic acid (11.77), Benzaldehyde,4-ethyl-(12.92), 4H-Pyran-4-one, 2,3 dihdro-3,5-dihydroxyl-6-methy-(6.89), Ethanone,1-(2-hydroxy-5-methylphenyl) (2.58), Methyl 4-(hydroxymethyl) benzoate (8.04), p-Menth-3-en-9-ol (3.73), Eugenol (1.44), Acetamide, N-tetrahydrofurfuryl-2-methoxy-(4.30), n-Hexadecanoic acid (12.64), 9,12-Octadecadienoic acid methy ester (1.03), Oleic acid (10.33), Octadecanoic acid (6.32) while the chromatograph of the aqueous extract of Tridax procumbens using GC-MS is shown in Figure 7. Table 2 Bioactive compounds detected in aqueous Tridax procumbens S/N Retention Time (min) Compound detected Chemical symbol Peak area (%) 1 8.00 2,3-Butanediol C4H10O2 10.05 2 9.46 Hexanoic acid C6H12O2 11.77 3 15.00 Benzaldehyde, 4-ethyl- C9H10O 12.92 4 16.62 4H-Pyran-4-one, 2,3-dihydro-3,5- dihydroxy-6-methyl- C6H8O4 6.89 5 21.00 Ethanone, 1-(2-hydroxy-5-methylphenyl)- C9H10O2 2.58 6 22.00 Methyl 4-(hydroxymethyl) Benzoate C9H10O3 8.04 7 22.50 p-Menth-3-en-9-ol C10H18O 3.73 8 24.51 Eugenol C10H12O2 1.44 9 26.00 Acetamide, N-tetrahydro furfuryl-2-methoxy- C8H15NO3 4.30 10 27.00 n-Hexadecanoic acid C16H32O2 12.64 11 29.57 9,12-Octadecadienoic acid methyl ester C19H34O2 1.03 12 30.84 Oleic acid C18H34O2 10.33 13 32.50 Octadecanoic acid C18H36O2 6.32
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