International Journal of Horticulture, 2026, Vol.16, No.2, 77-87 http://hortherbpublisher.com/index.php/ijh 81 2.8 Statistical analysis All the recorded data were arranged systematically treatment wise under three replications using Microsoft Excel version 16.89.1. To determine the significant result between the treatments, Analysis of variance (ANOVA) was carried out using R studio version 4.4.1 and DMRT was used for mean separation at 5% level of significance (p<0.05). 3 Results and Analysis 3.1 Germination percentage, mean germination time (MGT) and days to 50% germination (T50) The results on germination percentage, mean germination time (MGT) and days to 50% germination (T50) affected by different seed priming method are presented in Table 2. Germination percentage, mean germination time, and days to 50% germination were significantly affected by different seed priming techniques. Table 2 Effect of seed priming on germination percentage, mean germination time (MGT) and days to 50 % germination (T50) of cucumber (Cucumis sativus cv. Bhaktapur Local) in Syangja, Nepal, 2024 Treatments Germination parameters Germination percentage MGT (Days) T50 (Days) Control 73.34c 6.90a 6.83a Hot water (45C for 5 minutes) 88.00a 6.30b 6.00b GA3 100 ppm 84.00ab 6.22bc 6.00b GA3 200 ppm 81.34abc 6.34bc 6.00b KNO3 1% 80.67abc 6.06c 6.00b KNO3 3% 80.00abc 6.19bc 6.00b Cow urine 5% 77.34bc 6.27bc 6.00b Cow urine 10% 80.00abc 6.26bc 6.00b Vermiwash 10% 76.67bc 6.17bc 6.00b Vermiwash 20% 80.67abc 6.41b 6.00b CV(%) 5.52 1.98 0.75 LSD0.05 7.54 0.21 0.07 Grandmean 80.20 6.31 6.08 SEm() 2.55 0.07 0.02 F-test * *** *** Note: Mean within the column followed by the same letter/s are not significantly different at 5% level of significance by DMRT. * Significant at 5% (p<0.05), ** Significant at 1% (p<0.01), *** Significant at 0.1% (p<0.001), NS= non-significant at 5% (p>0.05), SEm= Standard Error of mean, LSD= Least significant difference, CV= Coefficient of variance, MGT= Mean germination time and T50= Days to 50% germination Significantly the highest germination percentage (88.00%) was found in hot water (45 C for 5 minutes). GA3 100 ppm (84.00%), GA3 200 ppm (81.34%), KNO3 1% (80.67%), Vermiwash 20% (80.67%), KNO3 3% (80.67%) and Cow urine 10% (80.00%), also showed increased germination percentage but were non-significant among themselves (LSD=7.54), while the lowest germination percentage (73.34%) was observed in control. Significantly the highest mean germination time was recorded in control (6.90 days), while the lowest MGT was found in KNO3 1% (6.06 days), which was not significantly different from Vermiwash 10% (6.17 days), KNO3 3% (6.19 days), GA3100 ppm (6.22 days), Cow urine 10% (6.26 days), Cow urine 5% (6.27 days) and GA3 200 ppm (6.34 days). Significantly the highest T50 (6.83 days) was found in control and the lowest T50 was found in hot water (6.00 days) which was not significantly different from Vermiwash 10% and 20%, Cow urine 5% and 10%, KNO3 1% and 3%, GA3 100 ppm and 200 ppm. 3.2 Seed vigour index (SVI-I and SVI-II) and speed of germination (BRI) The results on Seed vigour index I (SVI-I), Seed vigour index II (SVI-II) and speed of germination (BRI) are presented in Table 3. Significantly the highest SVI-I (2,643.83) was found in hot water (45 °C for 5 minutes)
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