Plant Gene and Trait 2025, Vol.16, No.1, 15-22 http://genbreedpublisher.com/index.php/pgt 20 enhance the accuracy and efficiency of breeding. In actual breeding, it is necessary to develop a cheaper and more practical genotyping system to make the MAS technology easier to be promoted and applied, so that both breeders and farmers can benefit better from this technology. Marker-assisted selection (MAS) has great potential in accelerating the breeding speed and improving the accuracy of target trait selection. Nowadays, DNA labeling technology and QTL localization technology are becoming increasingly advanced, providing breeders with powerful tools that can more effectively improve complex traits and enhance variety performance. As these technologies continue to develop, they are also expected to play an important role in addressing global food security issues and be used to cultivate new crop varieties that are high-yielding, stress-resistant and more nutritious, promoting agricultural progress. Combining molecular breeding with traditional methods can also make agriculture more efficient and sustainable. Acknowledgments Thank the Professor Liu for providing the research platform and support to ensure the smooth progress of this study. Conflict of Interest Disclosure The authors affirm that this research was conducted without any commercial or financial relationships that could be construed as a potential conflict of interest. References Barreto F., Rosa J., Balsalobre T., Pastina M., Silva R., Hoffmann H., De Souza A., Garcia A., and Carneiro M., 2019, A genome-wide association study identified loci for yield component traits in sugarcane (Saccharumspp.), PLoS One, 14(7): e0219843. https://doi.org/10.1371/journal.pone.0219843 Benchimol-Reis L., 2023, Molecular markers in plant breeding, Journal of Agricultural Science, 15(3): 58-84. https://doi.org/10.5539/jas.v15n3p58 Bonnett D., Rebetzke G., and Spielmeyer W., 2004, Strategies for efficient implementation of molecular markers in wheat breeding, Molecular Breeding, 15: 75-85. https://doi.org/10.1007/s11032-004-2734-5 Breseghello F., and Sorrells M., 2006, Association analysis as a strategy for improvement of quantitative traits in plants, Crop Science, 46(3): 1323-1330. https://doi.org/10.2135/cropsci2005.09-0305 Chung P., and Liao C., 2022, Selection of parental lines for plant breeding via genomic prediction, Frontiers in Plant Science, 13: 934767. https://doi.org/10.3389/fpls.2022.934767 De Los Campos G., Hickey J., Pong-Wong R., Daetwyler H., and Calus M., 2013, Whole-genome regression and prediction methods applied to plant and animal breeding, Genetics, 193: 327-345. https://doi.org/10.1534/genetics.112.143313 Fu Y., Yang M., Zeng F., and Biligetu B., 2017, Searching for an accurate marker-based prediction of an individual quantitative trait in molecular plant breeding, Frontiers in Plant Science, 8: 1182. https://doi.org/10.3389/fpls.2017.01182 Germanà M., 2011, Gametic embryogenesis and haploid technology as valuable support to plant breeding, Plant Cell Reports, 30: 839-857. https://doi.org/10.1007/s00299-011-1061-7 Godwin I., Rutkoski J., Varshney R., and Hickey L., 2019, Technological perspectives for plant breeding, Theoretical and Applied Genetics, 132: 555-557. https://doi.org/10.1007/s00122-019-03321-4 Gupta S., Goyal M., and Aggarwal N.K., 2017, Advances in molecular markers for plant breeding: applications in crop improvement, Plant Breeding Reviews, 41: 123-150. Hasan N., Choudhary S., Naaz N., Sharma N., and Laskar R., 2021, Recent advancements in molecular marker-assisted selection and applications in plant breeding programmes, Journal of Genetic Engineering and Biotechnology, 19(1): 128. https://doi.org/10.1186/s43141-021-00231-1 Jain S., Sharma D., and Kumar A., 2018, Genetic diversity and marker-assisted selection in forestry species: a focus on Sapindus, Tree Genetics and Genomes, 14(5): 65. https://doi.org/10.1007/s11295-018-0665-2 Jim F., 2024, Breeding 4.0: The breeding revolution of genetic information integration and editing, Molecular Plant Breeding, 15(1): 15-26. https://doi.org/10.5376/mpb.2024.15.0003 Kumawat G., Kumawat C., Chandra K., Pandey S., Chand S., Mishra U., Lenka D., and Sharma R., 2020, Insights into marker assisted selection and its applications in plant breeding, In: Abdurakhmonov I.Y. (ed.), Plant breeding- current and future views, IntechOpen, London, UK, pp.350. https://doi.org/10.5772/intechopen.95004 Li Z., Liu Y., and Wang Y., 2019, Recent developments in Sapindus species breeding and molecular applications, Industrial Crops and Products, 132: 17-26. https://doi.org/10.1016/j.indcrop.2019.01.013
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