Rice Genomics and Genetics 2024, Vol.15, No.6, 265-276 http://cropscipublisher.com/index.php/rgg 269 Figure 2 Effect of OsC1 overexpression in ABP and corresponding oxidative stress tolerance (Adopted from Upadhyaya et al., 2021). Image caption: (A) Semi-quantitative expression analysis of OsC1 in overexpressed rice plants along with NT and VT plants. ACTIN has been used as an internal reference control. (B) Total anthocyanin content estimation, (C) antioxidant activity measurement of isolated anthocyanin by DPPH radical scavenging assay of the OsC1-transformed (L1-L3), NT and VT plants (*P<0.01 and **P<0.001 show statistically significant differences with NT plants using one-way ANOVA followed by Dunnett's test). (D) Phenotypes of OsC1, VT, and NT seedlings grown under exogenously applied oxidative stress (10 μM MV) 3 and 5 days treatment. Unstressed nontransformed seedlings have been referred to as control (C). (E,F) Measurement of shoot length (E) and root elongation (F) of the OsC1-transformed, NT, and VT plants 3 and 5 days after oxidative stress application. At least five seedlings were measured for each individual parameters. (G-M) Effect of OsC1 overexpression and subsequent oxidative stress exposure in the transcript level of ABP structural genes. qRT-PCR expression analyses in seedlings before stress (BS) and after 3 days of stress (AS) for OsC1 gene (G) and the anthocyanin biosynthesis genes CHS (H), CHI (I), F3H(J), F3’H(K), DFR(L), ANS (M). The relative expression level of the genes in stressed NT and OsC1-transformed seedlings (L1, L2, and L3) were determined with respect to unstressed NT seedlings. ACTIN has been used an internal reference control. Data are represented as mean ± SD of three individual experiments (n=3 biological replicates, each containing 10 seedlings); *P<0.01 and ***P<0.0001 show statistically significant differences between before and after stress conditions of transformed plants using one-way ANOVA followed by Dunnett's test. Different alphabets above the bars represent significant differences (P<0.01) among the different transformed lines (i.e., L1, L2, and L3) and NT seedlings using one-way ANOVA followed by Tukey's HSD test. Alphabets without any prime represent the differences among seedlings before stress, while alphabets with primes (’) represents the differences among seedlings after stress (Adopted from Upadhyaya et al., 2021).
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