Cotton Genomics and Genetics 2025, Vol.16, No.6, 300-309 http://cropscipublisher.com/index.php/cgg 304 (Lei et al., 2022). In this way, the research and development of new varieties in response to market or environmental changes can proceed at a faster pace. Overall, this combination makes breeding no longer a "time war", but more like a precise regulation competition, fast, stable and with a clear direction. Figure 2 CLCrV-mediated simultaneous editing of GhCLA1 and GhPDS genes in cotton. (A) CLCrV-mediated design strategy for simultaneous editing of GhCLA1 and GhPDS. (B) Detection of GhPDS-sgRNA and GhCLA1-sgRNA1 targeted double mutations. Wild-type served as a control, and 1-7 were plant numbers. The gel image shows PCR products of the GhCLA1 gene and GhPDS gene, and digested PCR products with Pst I and Bfa I. The undigested PCR products lacking the Pst I and Bfa I site (due to the presence of a mutation) that were subsequently purified, cloned, and analyzed by sequencing. The red box indicates the double mutation of GhCLA1 and GhPDS. The green color indicates the PAM sequence. The restriction site on the target sequence is underlined in blue. M indicates the mutation sequence. Insertions are denoted with red capital letters. Deletions are shown as red dashes (Adopted from Lei et al., 2022) 5 Case Study in Place: Improving Fiber Quality Using Precision Tools 5.1 Background and breeding objective One of the most troublesome traits for researchers in cotton breeding is probably the quality of the fibers. Its genetic mechanism is complex and often goes against yield. When the fibers are better, the yield tends to drop (Zhao et al., 2024). This negative correlation has made improving the quality of upland cotton (Gossypium hirsutum) a long-standing problem.
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