Bioscience Methods 2025, Vol.16, No.1, 23-32 http://bioscipublisher.com/index.php/bm 30 of advanced breeding technologies like CRISPR-Cas9 and speed breeding should be expanded to accelerate the development of new cultivars. Finally, collaborative efforts between researchers, policymakers, and farmers are essential to ensure that the benefits of molecular breeding are widely disseminated and adopted, ultimately contributing to global food security and sustainable agriculture. Acknowledgments We are grateful to Dr. Huang for critically reading the manuscript and providing valuable feedback that improved the clarity of the text. We express our heartfelt gratitude to the two anonymous reviewers for their valuable comments on the manuscript. Conflict of Interest Disclosure The authors affirm that this research was conducted without any commercial or financial relationships that could be construed as a potential conflict of interest. References Ahmad D., Zhang Z., Rasheed H., Xu X., and Bao J., 2022, Recent advances in molecular improvement for potato tuber traits, International Journal of Molecular Sciences, 23(17): 9982. https://doi.org/10.3390/ijms23179982 Ahmar S., Gill R., Jung K., Faheem A., Qasim M., Mubeen M., and Zhou W., 2020, Conventional and molecular techniques from simple breeding to speed breeding in crop plants: recent advances and future outlook, International Journal of Molecular Sciences, 21(7): 2590. https://doi.org/10.3390/ijms21072590 Amundson K., Ordoñez B., Santayana M., Tan E., Henry I., Mihovilovich E., Bonierbale M., and Comai L., 2020, Genomic outcomes of haploid induction crosses in potato (Solanum tuberosumL.), Genetics, 214(2): 369-380. https://doi.org/10.1534/genetics.119.302843 Basu U., Bajaj D., Sharma A., Malik N., Daware A., Narnoliya L., Thakro V., Upadhyaya H., Kumar R., Tripathi S., Bharadwaj C., Tyagi A., and Parida S., 2018, Genetic dissection of photosynthetic efficiency traits for enhancing seed yield in chickpea, Plant, Cell and Environment, 42(1): 158-173. https://doi.org/10.1111/pce.13319 Caruana B., Pembleton L., Constable F., Rodoni B., Slater A., and Cogan N., 2019, Validation of genotyping by sequencing using transcriptomics for diversity and application of genomic selection in tetraploid potato, Frontiers in Plant Science, 10: 670. https://doi.org/10.3389/fpls.2019.00670 Dheer P., Rautela I., Sharma V., Dhiman M., Sharma A., Sharma N., and Sharma M., 2020, Evolution in crop improvement approaches and future prospects of molecular markers to CRISPR/Cas9 system, Gene, 753: 144795. https://doi.org/10.1016/j.gene.2020.144795 Ding N., Cui H., Miao Y., Tang J., Cao Q., and Luo Y., 2019, Single-molecule real-time sequencing identifies massive full-length cDNAs and alternative-splicing events that facilitate comparative and functional genomics study in the hexaploid crop sweet potato, PeerJ, 7: e7933. https://doi.org/10.7717/peerj.7933 Gebhardt C., 2013, Bridging the gap between genome analysis and precision breeding in potato, Trends in genetics, 29(4): 248-256. https://doi.org/10.1016/j.tig.2012.11.006 Gemenet D., Gemenet D., Lindqvist-Kreuze H., Boeck B., Pereira G., Pereira G., Mollinari M., Zeng Z., Yencho G., and Campos H., 2020, Sequencing depth and genotype quality: accuracy and breeding operation considerations for genomic selection applications in autopolyploid crops, Theoretical and Applied Genetics, 133: 3345-3363. https://doi.org/10.1007/s00122-020-03673-2 Guo H., Zhou M., Zhang G., He L., Yan C., Wan M., Hu J., He W., Zeng D., Zhu B., and Zeng Z., 2023, Development of homozygous tetraploid potato and whole genome doubling-induced the enrichment of H3K27ac and potentially enhanced resistance to cold-induced sweetening in tubers, Horticulture Research, 10(3): uhad017. https://doi.org/10.1093/hr/uhad017 Haque E., Shirasawa K., Suematsu K., Tabuchi H., Isobe S., and Tanaka M., 2023, Polyploid GWAS reveals the basis of molecular marker development for complex breeding traits including starch content in the storage roots of sweet potato, Frontiers in Plant Science, 14: 1181909. https://doi.org/10.3389/fpls.2023.1181909 Hasan N., Choudhary S., Naaz N., Sharma N., and Laskar R., 2021, Recent advancements in molecular marker-assisted selection and applications in plant breeding programmes, Journal of Genetic Engineering and Biotechnology, 19(1): 128. https://doi.org/10.1186/s43141-021-00231-1 He J., Zhao X., Laroche A., Lu Z., Liu H., and Li Z., 2014, Genotyping-by-sequencing (GBS), an ultimate marker-assisted selection (MAS) tool to accelerate plant breeding, Frontiers in Plant Science, 5: 484. https://doi.org/10.3389/fpls.2014.00484 Hirakawa H., Okada Y., Tabuchi H., Shirasawa K., Watanabe A., Tsuruoka H., Minami C., Nakayama S., Sasamoto S., Kohara M., Kishida Y., Fujishiro T., Kato M., Nanri K., Komaki A., Yoshinaga M., Takahata Y., Tanaka M., Tabata S., and Isobe S., 2015, Survey of genome sequences in a wild sweet potato, Ipomoea trifida (H. B. K.) G. Don, DNA Research, 22(2): 171-179. https://doi.org/10.1093/dnares/dsv002
RkJQdWJsaXNoZXIy MjQ4ODYzNA==