International Journal of Aquaculture, 2026, Vol.16, No.3, 184-195 http://www.aquapublisher.com/index.php/ija 188 shigelloides however shows only 40 isolates obtained from the selective agar were round-ended, straight rod shapes which are motile (15 isolates from experimental pond water and 25 from fish tissues (Table 3 and Table 4). 3.4 Morphological and biochemical characteristics of isolates of experimental pond water and fish tissues The 40 isolates further tested positive for oxidase, catalase, mannitol and citrate biochemical tests, they also tested negative for urease, methyl red and glucose biochemical tests (Table 5 and Table 6). Table 2 Total bacteria counts of experimental pond water and fish tissues (gill, liver and intestine) Weeks Pond 1 Pond 2 Pond 3 Pond 4 Pond 5 Pond 6 Water sample 0 5.70±0.00a 6.00±0.01a 5.60±0.02a 6.10±0.03a 6.00±0.02a 6.00±0.01a 2 7.00±0.02a 7.00±0.03a 7.00±0.01a 7.00±0.06 a 6.90±0.09a 7.00±0.03a 4 6.40±0.05a 6.80±0.05a 6.70±0.03a 6.80±0.08a 6.60±0.07a 6.70±0.02a 6 6.20±0.03ab 6.60±0.03b 6.50±0.04ab 6.00±0.03ab 5.80±0.01a 6.50±0.05ab 8 6.50±0.07a 6.70±0.06a 6.30±0.02a 6.60±0.05a 6.30±0.03a 6.60±0.07a Gill 0 6.60±0.03a 6.90±0.02a 6.70±0.00a 6.80±0.01a 6.80±0.07a 6.50±0.04a 4 7.00±0.05a 6.80±0.05a 6.90±0.05a 6.80±0.06a 6.60±0.04a 6.60±0.06a 8 6.50±0.02a 6.40±0.00 a 6.80±0.03a 6.60±0.02a 6.50±0.01a 6.50±0.02a Liver 0 6.90±0.04a 6.70±0.03a 6.80±0.05a 6.80±0.2a 6.70±0.05a 6.60±0.01a 4 6.80±0.02a 6.90±0.07a 7.00±0.08a 6.70±0.2a 6.60±0.03a 6.50±0.04a 8 6.50±0.00a 6.90±0.09a 6.90±0.04a 6.70±0.2a 6.60±0.06a 6.40±0.05a Intestine 0 6.50±0.01a 6.90±0.03a 6.80±0.07a 6.60±0.2a 6.50±0.00a 6.50±0.01a 4 6.80±0.02a 6.70±0.01a 7.00±0.09a 6.80±0.2a 6.70±0.04a 6.60±0.02a 8 6.80±0.04a 6.80±0.00a 6.80±0.03a 6.80±0.2a 6.70±0.03a 6.80±0.05a Means (n =2) in the same row with similar superscripts are not significantly different (p > 0.05) Table 3 Colony characteristics of isolates from experimental pond water Weeks Isolate code Colony shape Elevation Edge Surface Pigmentation Opacity 4 Dctr Short rod Raised Regular Smooth Pink Opaque Dctr Short rod Raised Regular Dull Pink Opaque DTii Short rod Raised Regular Smooth Pink Opaque DTii Short rod Raised Entire Smooth Pink Opaque DTiii Short rod Raised Regular Smooth Pink Opaque DTiv Short rod Raised Entire Smooth Pink Opaque DTv Short rod Raised Regular Smooth Pink Opaque DTvi Short rod Raised Regular Dull Pink Opaque DTvi Short rod Raised Irregular Rough Pink Opaque 8 Ectr Short rod Raised Regular Smooth Pink Opaque ETii Short rod Raised Regular Smooth Pink Opaque ETiii Short rod Raised Regular Smooth Pink Opaque ETiv Short rod Raised Regular Smooth Pink Opaque ETv Short rod Raised Entire Smooth Pink Opaque ETvi Short rod Raised Regular Dull Pink Opaque Keys: D, water sample at week 4; E, water sample at week 8; Ctr, experimental pond 1; Tii, experimental pond 2; Tiii, experimental pond 3; Tiv, experimental pond 4; Tv, experimental pond 5; Tvi, experimental pond 6; SR, Short rod
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