International Journal of Aquaculture, 2026, Vol.16, No.3, 156-165 http://www.aquapublisher.com/index.php/ija 160 depurated shellfish (Table 2 and Figure 3). The reductions in Faecal Coliform counts were highly significant across depuration intervals (F = 25.8, p < 0.01), with the most pronounced effects observed within the first 48 hours. Figure 3 FC reduction in V. cyprinoides during closed water depuration with a sponge filter 2.2.4 Reduction in Vibrio spp. count in the black clam Villorita cyprinoides The initial Vibrio spp. count was found to be 5.1x105 cfu/g. After 48 hours of depuration the Vibrio spp. count becomes 1.2x105 cfu/g (reduction around 76.47 %) (Table 2 and Figure 4). Complete depuration of Vibrio spp was not achieved within the 72 hours of depuration. Figure 4 Vibrio spp. reduction in V. cyprinoides during closed water depuration with a sponge filter 2.3 Bacteriological changes in biofilm-associated microbes on the depuration tank walls during depuration Bacterial load in the biofilm samples of the depuration tank wall at different depuration time intervals (0 h, 24 h, 48h, 72 h) was recorded for all parameters, including TC, FC, THB and Vibrio spp. Count (Table 3). The results showed
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