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分子植物育种
(
网络版
), 2014
,
12
,
1016
-
1023
Fenzi Zhiwu Yuzhong (Online), 2014, Vol.12, 1016
-
1023
http://mpb.5th.sophiapublisher.com
Copyright © 2014 5
th
Publisher 1016
研究论文
Research Article
开发
S5
功能标记应用于水稻广亲和品种筛选
吴爽
1*
,
台德卫
1*
,
宛煜嵩
2
,
王德正
1
,
张效忠
1
,
王守海
1
1.
安徽省农业科学院水稻研究所
,
合肥
, 230031
2.
中国农业科学院生物技术研究所
,
北京
, 100081
*
同等贡献作者
通讯作者:
wshouhai@s ina.com
作者
分子植物育种
, 2014
,
12
,
3
doi: 10.5376/mpb.cn.2014.12.0003
这是一篇采用
Creative Commons Attribution License
进行授权的开放取阅论文。只要对本原作有恰当的引用
,
版权所有人允许并同意第三方无条
件的使用与传播。
引用格式
(
中文
)
吴爽等
, 2014,
开发
S5
功能标记应用于水稻广亲和品种筛选
,
分子植物育种
(online), 12(3): 1016-1023 (doi: 10.5376/mpb.cn.2014.12.0003)
引用格式
(
英文
)
Wu et al., 2014, Developing Functional Marker for
S5
Gene and Screening Wide Compatibility Variety, Fenzi Zhiwu Yuzhong (online) (Molecular Plant
Breeding), 12(3): 1016-1023 (doi: 10.5376/mpb.cn.2014.12.0003)
广亲和基因
S5
能有效地提高水稻亚种间杂交种的结实率,被广泛地应用于育种实践,如本单位以广亲和品种
Cpslo17
S5
基因供体育成的双优
4183
,已通过品种审定并应用于生产。本研究利用公开的
S5
分子信息,开发出基于
PCR
的功能性标记,对安徽省水稻地方品种等材料进行筛选,并对
5
份材料进行测序。研究结果表明:一份来源于东南亚材料含
S5
基因,但
332
份安徽省水稻地方品种没有检测到
S5
基因,说明生殖隔离阻碍了基因交流,使得地方品种保留了遗传稳
定性和多样性,同时也说明了全面收集和保存地方品种的重要性。
关键词
广亲和品种
;
S5
;
功能性标记
;
筛选
Developing Functional Marker for
S5
Gene and Screening Wide
Compatibility Variety
Wu Shuang
1*
, Tai Dewei
1*
, Wan Yusong
2
, Wang Dezheng
1
, Zhang Xiaozhong
1
, Wang Shouhai
1
1. Rice Research Institute, Anhui Academy of Agricultural Sciences, Hefei, 230031, P.R. China
2. Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, P.R. China
*These workers contributed to this work
Corresponding author, wshouhai@sina.com;
Authors
Abstract
S5
is a wide compatibility gene, which can improve the seed setting rate of inter-subspecific hybrid
rice effectively and has been widely used in rice breeding programs. For example, Shuangyou 4183, which was
bred using Cpslo17 as the
S5
gene donor, has been registered and released by our group. A functional marker to
detect
S5
gene was developed using public available molecular information and it was used for screening
germplasm with wide compatibility among rice landraces in Anhui et al, and 5 rice varieties were sequenced.
Results indicated that one variety from Southeast Asia was identified with
S5
gene, but none of 332 rice landraces
in Anhui province was found with
S5
gene, which means that reproductive isolation prevents gene flow and
retains genetic stability and diversity in those rice landraces. On the other hand, extensively collecting and
protecting these rice landraces is very important for rice breeding and academic study.
Keywords
Wide compatibility variety;
S5
; Functional marker; Screening
研究背景
20
世纪
60
年代开始,随着全球人口的快速
增长及农业用地被大量占用,粮食短缺问题引起了
全球的广泛关注。为了满足对粮食的需求,到
2025
年粮食产量要在现有基础上
(1995
年全球谷物总产
收稿日期:
2014
01
23
接受日期:
2014
01
24
发表日期:
2014
03
23
基金项目:本研究由安徽省农业科学院院长青年创新基金
(11B10105)
和国家高技术研究发展计划
(2011AA10A100)
共同资助
分子植物育种
F
enzi Zhiwu Yuzhong