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分子植物育种
(
网络版
), 2013
,
11
,
1098
-
1105
Fenzi Zhiwu Yuzhong (Online), 2013, Vol.11, 1098
-
1105
http://mpb.5th.sophiapublisher.com
Copyright © 2013 5
th
Publisher 1098
研究报告
Article Report
棉花四大栽培种干旱胁迫下叶片蛋白质组分变化
张德超
,
阴祖军
,
王德龙
,
王俊娟
,
樊伟丽
,
王帅
,
孔静静
,
陆许可
,
叶武威
中国农业科学院棉花研究所
,
棉花生物学国家重点实验室
,
农业部棉花遗传改良重点开放实验室
,
安阳
, 455000
通讯作者:
yew158@163.com
作者
分子植物育种
, 2013
,
11
,
15
doi: 10.5376/mpb.cn.2013.11.0015
这是一篇采用
Creative Commons Attribution License
进行授权的开放取阅论文。只要对本原作有恰当的引用
,
版权所有人允许并同意第三方无条
件的使用与传播。
引用格式
(
中文
)
张德超等
, 2013,
棉花四大栽培种干旱胁迫下叶片蛋白质组分变化
,
分子植物育种
(online), 11(15): 1098-1105 (doi: 10.5376/mpb.cn.2013.11.0
0
15)
引用格式
(
英文
)
Zhang et al., 2013, The Effects of Drought Stress on Leaf Protein Components of the Four Cotton Cultivars, Fenzi Zhiwu Yuzhong (online) (Molecular
Plant Breeding), 11(15): 1098-1105 (doi: 10.5376/mpb.cn.2013.11.0015)
全球气候日益变暖,旱灾频繁发生,干旱严重影响棉花生产。
为了解棉花种质在干旱胁迫下叶片蛋白组分的
变化,选用棉花四大栽培种的抗旱和不抗旱材料
16
份,以棉花三叶期真叶为样品,优化棉花叶片蛋白组分提取方法,测
定正常浇水、短期干旱和长期干旱下叶片四种蛋白组分含量。
结果表明:棉花叶片各蛋白组分含量差异较大,谷蛋白含
量最高,清蛋白、球蛋白和醇溶蛋白含量较低;不同棉花栽培种叶片蛋白组分含量差异较大,四倍体棉叶片总蛋白含量
较高,清蛋白和谷蛋白含量高于二倍体棉,而二倍体棉叶片球蛋白和醇溶蛋白含量较高;干旱胁迫下,棉花叶片蛋白组
分含量会发生相应变化,不同栽培种、不同抗旱水平的品种蛋白组分的变化不同,表现出蛋白质代谢的多样性。
关键词
棉花
;
干旱胁迫
;
清蛋白
;
球蛋白
;
醇溶蛋白
;
谷蛋白
The Effects of Drought Stress on Leaf Protein Components of the Four Cotton
Cultivars
Zhang Dechao , Yin Zujun , Wang Delong , Wang Junjuan , Fan Weili , Wang Shuai , Kong Jingjing ,
Lu Xuke , Ye Wuwei
State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Key Laboratory for Cotton Genetic
Improvement, Ministry of Agriculture, Anyang , 455000, P.R. China
Corresponding author, yewu158@163.com;
Authors
Abstract
With the global warming, more and more frequent and serious drought stress has become one of the most leading
elements limiting the cotton production. 16 cotton accessions with drought-tolerant and drought-sensitive
spp.
from the four
cultivated species, were choosed to study the variations of protein components under drought stress. Of the tri-leaf stage of seedlings,
the protein extracted method of cotton leaves was optimized, to determine four kinds of protein under the stress of the control, mild
drought and heavy drought. The results indicated that there is a huge difference of protein level in different materials, Glutenin is the
most, Albumin, Globin and Prolamin are relatively low; The content of protein component in leaves had a great difference in
different cotton cultivars, the protein content in
G.hirsutum
and
G.barbadense
is relatively high, the content of Albumin and Globin is
higher than Diploid cotton, but Globin and Prolamin in Diploid cotton is relatively high, the variation of protein contents in different
cultivars, different drought resistance level changed greatly after drought stress, show the diversity of protein metabolism.
Keywords
Cotton; Drought stress; Albumin; Globin; Prolamin; Glutenin
研究背景
近年来环境恶化,全球气候变暖,淡水资源缺
乏导致世界各地旱情频繁发生,全球约有
43%
的耕
地处于干旱和半干旱地区,中国干旱和半干旱土地
总面积为
5.42×10
10
hm
2
,占全国总耕地面积的一半
以上,有些地区还遭受季节性干旱和难以预测的不
定期干旱
(
李智念等
, 2003)
。干旱已成为世界农业生
产面临的严峻挑战
棉花是中国乃至全世界重要的
收稿日期:
2013
03
12
接受日期:
2013
05
08
发表日期:
2013
05
29
基金项目:本研究由国家
973
重点基础研究发展计划
(2010CB126006)
资助