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分子植物育种
(
网络版
), 2013
,
11
,
1008
-
1013
Fenzi Zhiwu Yuzhong (Online), 2013, Vol.11, 1008
-
1013
http://mpb.5th.sophiapublisher.com
Copyright © 2013 5
th
Publisher
1008 
研究报告
Research Report
元阳梯田原位保护与种子库保存水稻地方品种的遗传多样性比较
唐有福
1,2
,
白筱
1
,
高东
1,3
,
何霞红
1
1.
云南农业大学农业生物多样性应用技术国家工程中心
,
昆明
, 650201
2.
元阳县新街镇农业综合服务中心
,
元阳
, 662416
3.
深圳大学
,
深圳
, 518060
通讯作者:
gaodong521@yahoo.com.cn;
作者
分子植物育种
, 2013
,
11
,
2
doi: 10.5376/mpb.cn.2013.11.0002
这是一篇采用
Creative Commons Attribution License
进行授权的开放取阅论文。只要对本原作有恰当的引用
,
版权所有人允许并同意第三方无条
件的使用与传播。
引用格式
(
中文
)
唐有福等
, 2013,
元阳梯田原位保护与种子库保存水稻地方品种的遗传多样性比较
,
分子植物育种
(online), 11(2): 1008-1013 (doi:
10.5376/mpb.cn.2013.11.0002)
引用格式
(
英文
)
Tang et al., 2013, Comparison of Genetic Diversity between
In-situ
and Seed Bank Conserved Rice Paddy Landraces in Yuanyang Terrace, Fenzi Zhiwu
Yuzhong (online) (Molecular Plant Breeding), 11(2): 1008-1013 (doi: 10.5376/mpb.cn.2013.11.0002)
为评估元阳梯田原位保护与种子库保存水稻
(
Oryza sativa
L.)
地方品种的遗传多样性变异状况,选用
24
SSR
引物
对元阳哈尼梯田
3
个原位保护品种和对应的
3
个种子库保护品种进行遗传多样性比较分析。共检测到
70
个等位基因,平均为
2.7
,变幅为
1~4
,原位保护聚群较种子库保护聚群多
8
个等位基因。原位保护聚群的各
SSR
位点等位基因数、有效等位基因
数、位点多态信息含量和基因型多样性等指标均高于种子库保护聚群,分别为
2.75>2.25 (
A
)
2.13>1.81 (
Ae
)
0.51>0.42(
PIC
)
0.84>0.62 (H′)
。聚类分析显示,供试
3
对水稻地方品种在
0.6610
的遗传相似性系数水平上聚在一起,
3
对原位和种子库保护聚
群分别在
0.9600
0.9322
0.9288
的相似系数上两两聚在一起,无交叉现象。结果暗示原位保护是维持种群遗传多样性的一种
更好的方式。
关键词
哈尼梯田;水稻地方品种;微卫星标记;原位保护;种子库保存
Comparison of Genetic Diversity between
In-situ
and Seed Bank Conserved Rice
Paddy Landraces in Yuanyang Terrace
Tang Youfu
1,2
, Bai Xiao
1
, Gao Dong
1,3
, He Xiahong
1
1. The National Center for Agricultural Biodiversity, Yunnan Agricultural University, Kunming, 650201, P.R., China
2. Agricultural Comprehensive Service Center of Xinjie Town, Yuanyang, 662416, P.R., China
3. Shenzhen University, Shenzhen, 518060, P.R., China
Corresponding author, gaodong521@yahoo.com.cn;
Authors
Abstract
To evaluate the genetic variation between
in-situ
and seed bank conserved rice paddy landraces in Yuanyang terrace,
the genetic diversity of 3 pair paddy rice landraces were analyzed by 24 SSR markers. The results showed that the mean alleles are
2.7 ranging from 1 to 4 of the detected total 70 alleles. Compared with the seed bank conserved samples, the in-situ conserved
samples were higher in the number of alleles, average number of alleles, effective number of alleles, locus polymorphism
information content, and genotype diversity, with
A
of 2.75>2.25,
Ae
of 2.13>1.81,
PIC
of 0.51>0.42, and
H'
of 0.84>0.62. An
UPGMA dendrogram based on the cluster analysis of genetic similarity showed that 3 pair paddy rice landraces clutered with the
similarity coefficients under 0.661. The similarity coefficients was 0.9600, 0.9322 and 0.9288 between Yuelianggu, Hongjiaolaojing
and Baijiaolaojing pairs, respectively. The results showed that
in-situ
conservation is the better way for maintaining genetic diversity
of populations of rice landraces.
Keywords
Hani terrace;
Oryza sativa
L. landraces; SSR marker;
In-situ
conservation; Seed bank conservation
研究背景
稻种资源是水稻优良基因的载体,蕴藏着包括
抗病虫、抗逆、高产、优质及其他一些优良的性状
基因
(
阮仁超等
, 2001)
。因此,切实保护现有种质资
收稿日期:
2013
01
09
接受日期:
2013
02
28
发表日期:
2013
04
15
基金项目:本研究由国家重点基础研究发展计划
(973
项目
)
课题
(2011CB100406)
资助