Page 15 - 2012no59

Basic HTML Version

分子植物育种
(
网络版
), 2012
,
10
,
1431
-
1437
Fenzi Zhiwu Yuzhong (Online), 2012, Vol.10, 1431
-
1437
http://mpb.5th.sophiapublisher.com
1431
研究报告
Research Article
水稻磷高效基因的初步定位
易俊良
1,2*
,
周德贵
2*
,
卢德城
2
,
吴耀荣
3
,
李宏
2
,
黄道强
2
,
赖穗春
2
,
王重荣
2
,
王志东
2
,
立云
1
,
谢旗
3
,
周少川
1,2
1.
湖南农业大学水稻科学研究所
,
长沙
, 410128
2.
广东省农业科学院水稻科学研究所
,
广州
, 510640
3.
中国科学院遗传与发育生物学研究所
,
北京
, 100101
*
同等贡献作者
通讯作者
:
qxie@genetics.ac.cn; xxs123@163.com
作者
分子植物育种
, 2012
,
10
,
59
doi: 10.5376/mpb.cn.2012.10.0059
收稿日期:
2012
07
11
接受日期:
2012
08
15
发表日期:
2012
12
15
本文首次发表在《分子植物育种》
(2012
年第
10
卷第
6
628-634
)
上。现依据版权所有人授权的许可协议,采用
Creative Commons Attribution
License
协议对其进行授权,再次发表与传播。只要对原作有恰当的引用
,
版权所有人允许并同意第三方无条件的使用与传播。
引用格式
(
中文
)
易俊良等
, 2012,
水稻磷高效基因的初步定位
,
分子植物育种
(online) Vol.10 No.59 pp.1431-1437 (doi: 10.5376/mpb. cn.2012.10.0059)
引用格式
(
英文
)
Yi et al., 2012, Molecular Mapping of Phosphorus-Efficient Gene in Rice, Fenzi Zhiwu Yuzhong (online) (Molecular Plant Breeding) Vol.10 No.59
pp.1431-1437 (doi: 10.5376/mpb.cn.2012.10.0059)
磷是植物生长发育所必需的大量营养元素。植物磷营养高效利用通常与根的形态、根分泌物、膜与体内磷转运以
及菌根等因素有关,表现为受多基因控制。本文通过前期筛选工作,获得
1
份耐低磷株系。利用
230
SSR
InDel
引物,
对低磷材料所在的回交导入系群体进行了初步定位,定位结果显示,与磷利用效率相关的基因座位有两个
OsPe5
OsPe7
分别位于第
5
染色体的
InDel520
InDel529
标记之间,与
InDel525
标记共分离,物理距离约为
900 kb
,和第
7
染色体
InDel703
InDel717
标记之间,与
InDel713
标记共分离,物理距离约为
1 400 kb
关键词
水稻
;
磷高效利用
;
近等基因导入系
;
基因定位
Molecular Mapping of Phosphorus-Efficient Gene in Rice
Yi Junliang
1,2*
, Liu Yang
2*
, Lu Decheng
2
, Wu Yaorong
3
, Li Hong
2
, Huang Daoqiang
2
, Lai Suichun
2
,
Wang Chongrong
2
, Wang Zhidong
2
, Chen Liyun
1
, Xie Qi
3
, Zhou Shaochuan
1,2
1. Rice Research Institute, Hunan Agricultural University, Changsha, 410128
2. Rice Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640
3. Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, P.R., China
* These authors contributed equally
Corresponding author, qxie@genetics.ac.cn; xxs123@163.com;
Authors
Abstract
Phosphorus (P) was an essential macronutrient for plant growth and development. High-efficient utilization of P-nut-
rition in plant was closely related to many factors, such as root morphology, root exudates, P-transportors across plasmalemma,
P-translocation and symbiosis with microorganisms and so on. These nutritional properties were genetically controlled by
multi-genes and characterized by quantitative trait loci (QTLs). In this study, a line with tolerance to low P was selected based on the
identification of previous work. Backcross introgression lines (BIL) population with low-phosphorus materials were used for
preliminary mapping by using 230 pairs of SSR and InDel primers. The results showed that two loci were associated with P
utilization efficiency and mapped between the markers of InDel520 and InDel529 on the chromosome 5 and InDel703 and InDel717
on the chromosome 7, respectively. The locus on the chromosome 5 was co-segregated with the InDel525, about 900 kb physical
distance, wher eas the locus on the chromosome 7 was co-segregated with the InDel713, approximately 1 400 kb physical distance
Keywords
Rice; High-efficient phosphorus utilization; Near-isogene introgression lines; Gene mapping
番磷高效利用性状是绿色超级稻
(Green super
rice)
的重要技术指标
(
张启发
, 2005; Zhang, 2007)
绿色超级稻的基本技术路线是通过转基因技术及
分子标记辅助选择技术与常规育种技术相结合,培
育营养高效、抗病虫害、抗逆性强的水稻超级稻新
品种,实现少施肥、少打药或基本不打药、节水抗
旱、优质高产的综合目标。水稻磷利用的田间筛选
指标一般采用生物产量、经济产量、根干重、根冠
比、分蘖数、有效穗、磷吸收效率、磷运输效率、磷
利用效率等评价指标
(
明凤等
, 1999;
汤翠凤等
,