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分子植物育种
(
网络版
), 2012
,
10
,
1218
-
1226
Fenzi Zhiwu Yuzhong (Online), 2012, Vol.10, 1218
-
1226
http://mpb.5th.sophiapublisher.com
1218
评述与展望
Review and Progress
根癌农杆菌介导的甘薯遗传转化影响因素研究进展
闫会
1
,
李强
1,2
1.
中国农业科学院甘薯研究所,中国农业科学院甘薯遗传改良重点开放实验室
,
徐州
, 221131
2.
江苏徐州甘薯研究中心,农业部甘薯生物学与遗传育种重点实验室
,
徐州
, 221131
通讯作者
:
instrong@163.com
作者
分子植物育种
, 2012
,
10
,
31
doi: 10.5376/mpb.cn.2012.10.0031
收稿日期:
2012
04
25
接受日期:
2012
05
15
发表日期:
2012
06
13
这是一篇采用
Creative Commons Attribution License
进行授权的开放取阅论文。只要对本原作有恰当的引用
,
版权所有人允许并同意第三方无条
件的使用与传播。
引用格式
(
中文
)
闫会
,
李强
, 2012,
根癌农杆菌介导的甘薯遗传转化影响因素研究进展
,
分子植物育种
(online) Vol.10 No.31 pp.1218-1226 (doi:
10.5376/mpb.cn.2012.10.0031)
引用格式
(
英文
)
Yan and Li, 2012, Research progress of factors affecting genetic transformation of sweetpotato mediated by Agrobacterium tumefaciens, Fenzi Zhiwu
Yuzhong (online) (Molecular Plant Breeding) Vol.10 No.31 pp.1218-1226 (doi: 10.5376/mpb.cn.2012.10.0031)
甘薯是世界上重要的块根作物,是生产动物饲料、淀粉和燃料乙醇的重要原料。甘薯为异源六倍体,并且自交不
亲和,因此难以通过常规育种方法聚合优良性状。甘薯遗传转化体系的建立为甘薯转基因分子育种提供了技术手段,使得高
产、优质、耐逆等优异基因的利用更加高效。在甘薯遗传转化中,应用最广泛的是根癌农杆菌介导法。本文探讨了根癌农杆
菌介导的甘薯遗传转化影响因素,主要包括遗传因素和环境因素两大类。其中遗传因素有菌株类型,菌液浓度,甘薯基因型,
转化受体类型,外植体状态等;环境因素包括诱导物种类,如酚类化合物、糖类、硝酸银等,共培养时间,选择压,选择方
法,侵染时间等。同时概述了有益性状基因转化甘薯的现状,展望了甘薯遗传转化未来发展方向和应用前景。
关键词
甘薯
;
根癌农杆菌
;
遗传转化
;
影响因素
Research progress of factors affecting genetic transformation of sweetpotato
mediated by
Agrobacterium tumefaciens
Yan Hui
1
, Li Qing
1,2
1. Sweetpotato Research Institute, Key Laboratory of Sweetpotato Genetic Improvement, Chinese Academy of Agricultural Sciences, Xuzhou, 221131
2. Jiangsu Xuzhou Sweetpotato Research Center, Key Laboratory of Biology and Genetic Improvement of Sweetpotato, Ministry of Agriculture, Xuzhou,
221131
Corresponding author, instrong@163.com;
Authors
Abstract
Sweetpotato is an important root crop and serves as raw material for the processing of feed, starch and biofuel in various
industries. Conventional breeding method has a relatively low efficiency because of its allohexaploid and self-incompatibility.
Establishment of efficient genetic transformation system provides an essential tool for sweetpotato improvement. It shortens breeding
periods, and realizes resource efficient utilization, such as high yield, good quality and stress tolerance genes. Recently,
Agrobacterium
-mediated genetic transformation is widely used genetically modified method for sweetpotato. We discussed the
factors affecting the genetic transformation mediated by
Agrobacterium -tumefaciens
in sweetpotato, including genetic factors and
environmental factors. Genetic factors include strain type, bacteria liquid concentration, sweetpotato genotype and acceptance
materials, explant state, etc., and the later include species of induction, training time, pressure selection, the choice method, infection
time, etc. The inductor sinclude phenolic compounds, sugar, nitric acid silver and so on. We also summarized the current status of
beneficial genes used in sweetpotato, and visualized the direction of sweet potato genetic transformation and its prospects in the
future.
Keywords
Sweetpotato,
Agrobacterium tumfaciens
, Genetic transformation, Affecting factors
甘薯是世界上重要的粮食、工业原料和新型生
物能源作物。据统计,我国甘薯栽培面积和产量分
别约占世界的
50%
80% (FAO, 2010, http://faostat.
fao.org/site/567/DesktopDefault.aspx?PageID=567#an
cor)
,是世界上最大的甘薯生产国。因此,中国甘
薯产业发展具有全球意义。长期以来,常规育种是
甘薯品种改良的主要途径,但是由于甘薯染色体遗
传背景复杂及自交不亲和等特点
(
李强等
, 2005b)